Journal: Journal of Cellular and Molecular Medicine

Article Title: Novel long non‐coding RNA CYB561‐5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non‐small cell lung cancer

doi: 10.1111/jcmm.17057

Figure Lengend Snippet: Lnc‐CYB561‐5 promotes aerobic glycolysis in vivo. (A) Cluster analysis of differentially expressed mRNAs in H1299 cells treated with the lnc‐CYB561‐5 knockdown. (B) The top 20 GO enrichment of significantly upregulated genes (fold change > 2, p < 0.05, top 100). (C) Heatmap depicting relative expression of known genes related to glycolysis. (D and E) Measurement of ECARO and CR in H1299 cells treated with the lnc‐CYB561‐5 knockdown. (F) Relative glycolysis rates in H1299 cells, as judged by Seahorse analyses. (G–I) Relative mRNA and protein expression levels of Pfk1, Hk1, Hk2, Eno1, G6pi and Glut1 in H1299 cells. * p < 0.05, ** p < 0.01, *** p < 0.001, NS, p > 0.05 vs. the sh‐Ctrl group, n = 5. Data are presented as means ± SEM. Multiple group comparisons were performed using one‐way ANOVA followed by Tukey's post hoc test

Article Snippet: After blocking with 5% non‐fat milk for 1 h at room temperature, the samples were incubated with the corresponding primary antibody against Pfk1 (Affinity, Cat No. DF7362; 1:1000), G6pi (Affinity, Cat No. DF13660; 1:500), HK1 (Proteintech, Cat No. 15656–1‐AP), HK2 (Proteintech, Cat No. 66974–1‐Ig), Eno1 (Affinity, Cat No. DF2920; 1:1000), Glut1 (CST, Cat No. 12939), Bsg (Proteintech, Cat No. 11989–1‐AP), Lbr (Proteintech, Cat No. 12398–1‐AP) and β‐catenin (Proteintech, Cat No. 51067–2‐AP, 1:1000) at 4°C for 12 h. Then, the membranes were incubated with the corresponding fluorescently labelled secondary antibody, including anti‐mouse or anti‐rabbit antibodies (Li‐Cor Biosciences) for 2 h at room temperature.

Techniques: In Vivo, Knockdown, Expressing

Journal: Cell reports

Article Title: CPSF1 inhibition promotes widespread use of intergenic polyadenylation sites and impairs glycolysis in prostate cancer cells

doi: 10.1016/j.celrep.2024.115211

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies were purchased from Santa Cruz for western blot detection of CPSF1, (G-10, sc-166281, 1:100) and tubulin (B-5-1-2, sc-23948, 1:3000), Proteintech for western blot detection of NUDT21 (66335-1-Ig, 1:500) and PCF11 (23540-1-AP, 1:500), Fortis Life Sciences for western blot detection of CPSF6 (A301-356A, 1:1,000) and Origene for western blot detection of GPI (TA501171, 1:500).

Techniques: Recombinant, shRNA, Over Expression, Plasmid Preparation, Cloning, Virus, Software

Journal: International Journal of Molecular Sciences

Article Title: Evodiamine Induces Apoptosis and Inhibits Migration of HCT-116 Human Colorectal Cancer Cells

doi: 10.3390/ijms161126031

Figure Lengend Snippet: Migration ability, expression of PGI/AMF, JAK2/STAT3, and MMP3 were detected in HCT-116 cells treated with EVO. ( A ) Migration potential was assessed by Transwell assay after cells were treated by agents. Cells were seeded in Transwell insert upper part incubated with 10 μg/mL PGI/AMF ( b ) and with 10 μg/mL PGI/AMF + 6 μmol/L EVO ( c ) for 28 h, normal cells as the control group ( a ) (original magnification, 200×); ( B ) AMF was detected by ELISA after cells were treated with various concentrations of EVO for 6 h; ( C – F ) Cells were incubated with 6 μmol/L EVO for 2, 4, and 6 h, and the expression of PGI, MMP3 and JAK2/STAT3 was assessed. Relative expression ratio was calculated with Gray-scan value for ( D , F ). Data represent the results of three independent experiments (mean ± SD). Statistically different compared with the control (0 μmol/L of EVO) ( * p < 0.05; ** p < 0.01).

Article Snippet: The human GPI ELISA kit was purchased from Cusabio (Wuhan, China).

Techniques: Migration, Expressing, Transwell Assay, Incubation, Control, Enzyme-linked Immunosorbent Assay